Determination of sodium cromoglycate in human plasma by liquid chromatography–mass spectrometry in the turbo ion spray mode
Identifieur interne : 000092 ( France/Analysis ); précédent : 000091; suivant : 000093Determination of sodium cromoglycate in human plasma by liquid chromatography–mass spectrometry in the turbo ion spray mode
Auteurs : M. L Ozoux [France] ; J. Girault [France] ; J. M Malgouyat [France] ; O. Pasquier [France]Source :
- Journal of Chromatography B: Biomedical Sciences and Applications [ 0378-4347 ] ; 2001.
Abstract
A highly sensitivity liquid chromatography–tandem mass spectrometry method has been developed for the quantitation of sodium cromoglycate (SCG) in human plasma. The method was validated over a linear range of 0.100–50.0 ng/ml, using 13C4 sodium cromoglycate as the internal standard. Compounds were extracted from 1.0 ml of lithium heparin plasma by methanol elution of C18 solid-phase extraction cartridges. The dried residue was reconstituted with 100 μl of 0.01 N HCl, and 30 μl was injected onto the LC–MS–MS system. Chromatographic separation was achieved on a C8 (3.5 μm) column with an isocratic mobile phase of methanol–water–0.5 M ammonium acetate (35:64.8:0.2, v/v/v). The analytes were detected with a PE Sciex API 3000 mass spectrometer using turbo ion spray with positive ionization. Ions monitored in the multiple reaction monitoring (MRM) mode were m/z 469.2 (precursor ion) to m/z 245.1 (product ion) for SCG and m/z 473.2 (precursor ion) to m/z 247.1 (product ion) for 13C4 SCG (I.S.). The average recoveries of SCG and the I.S. from human plasma were 91 and 87%, respectively. The low limit of quantitation was 0.100 ng/ml. Results from a 4-day validation study demonstrated excellent precision (C.V.% values were between 1.9 and 6.5%) and accuracy (−5.4 to −1.2%) across the calibration range of 0.100–50.0 ng/ml.
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DOI: 10.1016/S0378-4347(01)00420-0
Affiliations:
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Pasquier</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A6872473A532279445576BE5E45698E69AA31364</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1016/S0378-4347(01)00420-0</idno><idno type="url">https://api.istex.fr/document/A6872473A532279445576BE5E45698E69AA31364/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000872</idno><idno type="wicri:Area/Istex/Curation">000705</idno><idno type="wicri:Area/Istex/Checkpoint">001D97</idno><idno type="wicri:doubleKey">0378-4347:2001:Ozoux M:determination:of:sodium</idno><idno type="wicri:Area/Main/Merge">002613</idno><idno type="wicri:Area/Main/Curation">002560</idno><idno type="wicri:Area/Main/Exploration">002560</idno><idno type="wicri:Area/France/Extraction">000092</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Determination of sodium cromoglycate in human plasma by liquid chromatography–mass spectrometry in the turbo ion spray mode</title><author><name sortKey="Ozoux, M L" sort="Ozoux, M L" uniqKey="Ozoux M" first="M. L" last="Ozoux">M. L Ozoux</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Department of Drug Metabolism and Pharmacokinetics (DMPK France), Aventis Pharma, 20 avenue Raymond Aron, F-92165 Antony Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Antony</settlement></placeName></affiliation><affiliation><wicri:noCountry code="no comma">Corresponding author. Tel.: +33-1-5571-7119; fax: 33-1-5571-6322</wicri:noCountry></affiliation><affiliation><wicri:noCountry code="no comma">E-mail: marie-laure.ozoux@aventis.com</wicri:noCountry></affiliation></author><author><name sortKey="Girault, J" sort="Girault, J" uniqKey="Girault J" first="J" last="Girault">J. Girault</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Bioanalytical Laboratories, Parexel-Cemaf, 6 avenue Mozart, F-86000 Poitiers</wicri:regionArea><placeName><region type="region" nuts="2">Aquitaine-Limousin-Poitou-Charentes</region><region type="old region" nuts="2">Poitou-Charentes</region><settlement type="city">Poitiers</settlement></placeName></affiliation></author><author><name sortKey="Malgouyat, J M" sort="Malgouyat, J M" uniqKey="Malgouyat J" first="J. M" last="Malgouyat">J. M Malgouyat</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Bioanalytical Laboratories, Parexel-Cemaf, 6 avenue Mozart, F-86000 Poitiers</wicri:regionArea><placeName><region type="region" nuts="2">Aquitaine-Limousin-Poitou-Charentes</region><region type="old region" nuts="2">Poitou-Charentes</region><settlement type="city">Poitiers</settlement></placeName></affiliation></author><author><name sortKey="Pasquier, O" sort="Pasquier, O" uniqKey="Pasquier O" first="O" last="Pasquier">O. Pasquier</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Department of Drug Metabolism and Pharmacokinetics (DMPK France), Aventis Pharma, 20 avenue Raymond Aron, F-92165 Antony Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Antony</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Chromatography B: Biomedical Sciences and Applications</title><title level="j" type="abbrev">CHROLD</title><idno type="ISSN">0378-4347</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">765</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="179">179</biblScope><biblScope unit="page" to="185">185</biblScope></imprint><idno type="ISSN">0378-4347</idno></series><idno type="istex">A6872473A532279445576BE5E45698E69AA31364</idno><idno type="DOI">10.1016/S0378-4347(01)00420-0</idno><idno type="PII">S0378-4347(01)00420-0</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-4347</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A highly sensitivity liquid chromatography–tandem mass spectrometry method has been developed for the quantitation of sodium cromoglycate (SCG) in human plasma. The method was validated over a linear range of 0.100–50.0 ng/ml, using 13C4 sodium cromoglycate as the internal standard. Compounds were extracted from 1.0 ml of lithium heparin plasma by methanol elution of C18 solid-phase extraction cartridges. The dried residue was reconstituted with 100 μl of 0.01 N HCl, and 30 μl was injected onto the LC–MS–MS system. Chromatographic separation was achieved on a C8 (3.5 μm) column with an isocratic mobile phase of methanol–water–0.5 M ammonium acetate (35:64.8:0.2, v/v/v). The analytes were detected with a PE Sciex API 3000 mass spectrometer using turbo ion spray with positive ionization. Ions monitored in the multiple reaction monitoring (MRM) mode were m/z 469.2 (precursor ion) to m/z 245.1 (product ion) for SCG and m/z 473.2 (precursor ion) to m/z 247.1 (product ion) for 13C4 SCG (I.S.). The average recoveries of SCG and the I.S. from human plasma were 91 and 87%, respectively. The low limit of quantitation was 0.100 ng/ml. Results from a 4-day validation study demonstrated excellent precision (C.V.% values were between 1.9 and 6.5%) and accuracy (−5.4 to −1.2%) across the calibration range of 0.100–50.0 ng/ml.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Aquitaine-Limousin-Poitou-Charentes</li><li>Poitou-Charentes</li><li>Île-de-France</li></region><settlement><li>Antony</li><li>Poitiers</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Ozoux, M L" sort="Ozoux, M L" uniqKey="Ozoux M" first="M. L" last="Ozoux">M. L Ozoux</name></region><name sortKey="Girault, J" sort="Girault, J" uniqKey="Girault J" first="J" last="Girault">J. Girault</name><name sortKey="Malgouyat, J M" sort="Malgouyat, J M" uniqKey="Malgouyat J" first="J. M" last="Malgouyat">J. M Malgouyat</name><name sortKey="Pasquier, O" sort="Pasquier, O" uniqKey="Pasquier O" first="O" last="Pasquier">O. 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